wild type c57bl 6j strain Search Results


primary cortical mouse neurons  (Lonza)
90
Lonza primary cortical mouse neurons
Primary Cortical Mouse Neurons, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wild+type+c57bl+6j+strain/pmc03096744-205-0-6?v=Lonza
Average 90 stars, based on 1 article reviews
primary cortical mouse neurons - by Bioz Stars, 2026-08
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c57 bl 6 j mice  (Vital River Laboratories)
86
Vital River Laboratories c57 bl 6 j mice
C57 Bl 6 J Mice, supplied by Vital River Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wild+type+c57bl+6j+strain/pmc12318516-81-5-15?v=Vital+River+Laboratories
Average 86 stars, based on 1 article reviews
c57 bl 6 j mice - by Bioz Stars, 2026-08
86/100 stars
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adult male c57bl 6j mice  (Vital River Laboratories)
86
Vital River Laboratories adult male c57bl 6j mice
Adult Male C57bl 6j Mice, supplied by Vital River Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wild+type+c57bl+6j+strain/pm41077082-34-0-18?v=Vital+River+Laboratories
Average 86 stars, based on 1 article reviews
adult male c57bl 6j mice - by Bioz Stars, 2026-08
86/100 stars
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rd1 mice  (Johns Hopkins HealthCare)
90
Johns Hopkins HealthCare rd1 mice
( A ) Schematic of Pde6b gene correction by Cas9/RecA in <t>rd1</t> (top) and wild-type (bottom) mice. The Pde6b point mutation (C to A) is marked in red, and the premature stop codon of the Pde6b rd1 mutation is labeled by a red arrowhead. The truncated rd1 protein is in red, and the wild-type sequence loss in the rd1 mutant is in blue. ( B ) Experimental design for Cas9/RecA-mediated HDR gene correction of Pde6b rd1 mutant mice. Plasmids required for Cas9/RecA were introduced into mouse retinae by retinal electroporation. ( C ) Schematic of genomic DNA and cDNA sequencing of Cas9/RecA-treated cells. EGFP + cells from treated rd1 mice were collected using glass capillaries, with DNA extracted and enriched by Dde I restricted enzyme digestion for genome sequencing or cDNA collected through Smart-seq2 methods. ( D ) Representative direct genomic sequencing results from Cas9/RecA-mediated HDR gene correction. The Cas9-treated group (top) did not detect any gene correction. The Cas9/RecA-treated group (bottom) showed A to C conversion (red dashed frame). ( E ) Representative sequencing result of Pde6b cDNA from the Cas9/RecA-treated group. A was converted to C by Cas9/RecA-mediated gene correction. ( F ) Representative Western blot results from Cas9/RecA-mediated gene correction, and quantification of levels of PDE6B protein. Western blot for PDE6B shows the recovery of PDE6B expression. Actin was used as a loading control. Two retinae were mixed together for each Western blot sample, and three independent experiments were done. 1/10 wild type, loading 1 / 10 volume wild type and 9 / 10 sample buffer to make up the volume; Cas9, rd1 mice treated with Cas9, sgRNA, and donor; Cas9/RecA, rd1 mice treated with Cas9, sgRNA, donor, and RecA.
Rd1 Mice, supplied by Johns Hopkins HealthCare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wild+type+c57bl+6j+strain/pmc06469935-160-1-14?v=Johns+Hopkins+HealthCare
Average 90 stars, based on 1 article reviews
rd1 mice - by Bioz Stars, 2026-08
90/100 stars
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b cell isolation kit  (Miltenyi Biotec)
96
Miltenyi Biotec b cell isolation kit
( A ) Schematic of Pde6b gene correction by Cas9/RecA in <t>rd1</t> (top) and wild-type (bottom) mice. The Pde6b point mutation (C to A) is marked in red, and the premature stop codon of the Pde6b rd1 mutation is labeled by a red arrowhead. The truncated rd1 protein is in red, and the wild-type sequence loss in the rd1 mutant is in blue. ( B ) Experimental design for Cas9/RecA-mediated HDR gene correction of Pde6b rd1 mutant mice. Plasmids required for Cas9/RecA were introduced into mouse retinae by retinal electroporation. ( C ) Schematic of genomic DNA and cDNA sequencing of Cas9/RecA-treated cells. EGFP + cells from treated rd1 mice were collected using glass capillaries, with DNA extracted and enriched by Dde I restricted enzyme digestion for genome sequencing or cDNA collected through Smart-seq2 methods. ( D ) Representative direct genomic sequencing results from Cas9/RecA-mediated HDR gene correction. The Cas9-treated group (top) did not detect any gene correction. The Cas9/RecA-treated group (bottom) showed A to C conversion (red dashed frame). ( E ) Representative sequencing result of Pde6b cDNA from the Cas9/RecA-treated group. A was converted to C by Cas9/RecA-mediated gene correction. ( F ) Representative Western blot results from Cas9/RecA-mediated gene correction, and quantification of levels of PDE6B protein. Western blot for PDE6B shows the recovery of PDE6B expression. Actin was used as a loading control. Two retinae were mixed together for each Western blot sample, and three independent experiments were done. 1/10 wild type, loading 1 / 10 volume wild type and 9 / 10 sample buffer to make up the volume; Cas9, rd1 mice treated with Cas9, sgRNA, and donor; Cas9/RecA, rd1 mice treated with Cas9, sgRNA, donor, and RecA.
B Cell Isolation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wild+type+c57bl+6j+strain/us11185548-1482-13-18?v=Miltenyi+Biotec
Average 96 stars, based on 1 article reviews
b cell isolation kit - by Bioz Stars, 2026-08
96/100 stars
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male c57bl 6 j mice  (Selleck Chemicals)
93
Selleck Chemicals male c57bl 6 j mice
( A ) Schematic of Pde6b gene correction by Cas9/RecA in <t>rd1</t> (top) and wild-type (bottom) mice. The Pde6b point mutation (C to A) is marked in red, and the premature stop codon of the Pde6b rd1 mutation is labeled by a red arrowhead. The truncated rd1 protein is in red, and the wild-type sequence loss in the rd1 mutant is in blue. ( B ) Experimental design for Cas9/RecA-mediated HDR gene correction of Pde6b rd1 mutant mice. Plasmids required for Cas9/RecA were introduced into mouse retinae by retinal electroporation. ( C ) Schematic of genomic DNA and cDNA sequencing of Cas9/RecA-treated cells. EGFP + cells from treated rd1 mice were collected using glass capillaries, with DNA extracted and enriched by Dde I restricted enzyme digestion for genome sequencing or cDNA collected through Smart-seq2 methods. ( D ) Representative direct genomic sequencing results from Cas9/RecA-mediated HDR gene correction. The Cas9-treated group (top) did not detect any gene correction. The Cas9/RecA-treated group (bottom) showed A to C conversion (red dashed frame). ( E ) Representative sequencing result of Pde6b cDNA from the Cas9/RecA-treated group. A was converted to C by Cas9/RecA-mediated gene correction. ( F ) Representative Western blot results from Cas9/RecA-mediated gene correction, and quantification of levels of PDE6B protein. Western blot for PDE6B shows the recovery of PDE6B expression. Actin was used as a loading control. Two retinae were mixed together for each Western blot sample, and three independent experiments were done. 1/10 wild type, loading 1 / 10 volume wild type and 9 / 10 sample buffer to make up the volume; Cas9, rd1 mice treated with Cas9, sgRNA, and donor; Cas9/RecA, rd1 mice treated with Cas9, sgRNA, donor, and RecA.
Male C57bl 6 J Mice, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wild+type+c57bl+6j+strain/pmc11264750-38-5-17?v=Selleck+Chemicals
Average 93 stars, based on 1 article reviews
male c57bl 6 j mice - by Bioz Stars, 2026-08
93/100 stars
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c57bl/6j mice  (Cyagen Biosciences)
90
Cyagen Biosciences c57bl/6j mice
( A ) Schematic of Pde6b gene correction by Cas9/RecA in <t>rd1</t> (top) and wild-type (bottom) mice. The Pde6b point mutation (C to A) is marked in red, and the premature stop codon of the Pde6b rd1 mutation is labeled by a red arrowhead. The truncated rd1 protein is in red, and the wild-type sequence loss in the rd1 mutant is in blue. ( B ) Experimental design for Cas9/RecA-mediated HDR gene correction of Pde6b rd1 mutant mice. Plasmids required for Cas9/RecA were introduced into mouse retinae by retinal electroporation. ( C ) Schematic of genomic DNA and cDNA sequencing of Cas9/RecA-treated cells. EGFP + cells from treated rd1 mice were collected using glass capillaries, with DNA extracted and enriched by Dde I restricted enzyme digestion for genome sequencing or cDNA collected through Smart-seq2 methods. ( D ) Representative direct genomic sequencing results from Cas9/RecA-mediated HDR gene correction. The Cas9-treated group (top) did not detect any gene correction. The Cas9/RecA-treated group (bottom) showed A to C conversion (red dashed frame). ( E ) Representative sequencing result of Pde6b cDNA from the Cas9/RecA-treated group. A was converted to C by Cas9/RecA-mediated gene correction. ( F ) Representative Western blot results from Cas9/RecA-mediated gene correction, and quantification of levels of PDE6B protein. Western blot for PDE6B shows the recovery of PDE6B expression. Actin was used as a loading control. Two retinae were mixed together for each Western blot sample, and three independent experiments were done. 1/10 wild type, loading 1 / 10 volume wild type and 9 / 10 sample buffer to make up the volume; Cas9, rd1 mice treated with Cas9, sgRNA, and donor; Cas9/RecA, rd1 mice treated with Cas9, sgRNA, donor, and RecA.
C57bl/6j Mice, supplied by Cyagen Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wild+type+c57bl+6j+strain/pm38097561-226-16-21?v=Cyagen+Biosciences
Average 90 stars, based on 1 article reviews
c57bl/6j mice - by Bioz Stars, 2026-08
90/100 stars
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male c57bl/6j mice  (clea japan inc)
90
clea japan inc male c57bl/6j mice
( A ) Schematic of Pde6b gene correction by Cas9/RecA in <t>rd1</t> (top) and wild-type (bottom) mice. The Pde6b point mutation (C to A) is marked in red, and the premature stop codon of the Pde6b rd1 mutation is labeled by a red arrowhead. The truncated rd1 protein is in red, and the wild-type sequence loss in the rd1 mutant is in blue. ( B ) Experimental design for Cas9/RecA-mediated HDR gene correction of Pde6b rd1 mutant mice. Plasmids required for Cas9/RecA were introduced into mouse retinae by retinal electroporation. ( C ) Schematic of genomic DNA and cDNA sequencing of Cas9/RecA-treated cells. EGFP + cells from treated rd1 mice were collected using glass capillaries, with DNA extracted and enriched by Dde I restricted enzyme digestion for genome sequencing or cDNA collected through Smart-seq2 methods. ( D ) Representative direct genomic sequencing results from Cas9/RecA-mediated HDR gene correction. The Cas9-treated group (top) did not detect any gene correction. The Cas9/RecA-treated group (bottom) showed A to C conversion (red dashed frame). ( E ) Representative sequencing result of Pde6b cDNA from the Cas9/RecA-treated group. A was converted to C by Cas9/RecA-mediated gene correction. ( F ) Representative Western blot results from Cas9/RecA-mediated gene correction, and quantification of levels of PDE6B protein. Western blot for PDE6B shows the recovery of PDE6B expression. Actin was used as a loading control. Two retinae were mixed together for each Western blot sample, and three independent experiments were done. 1/10 wild type, loading 1 / 10 volume wild type and 9 / 10 sample buffer to make up the volume; Cas9, rd1 mice treated with Cas9, sgRNA, and donor; Cas9/RecA, rd1 mice treated with Cas9, sgRNA, donor, and RecA.
Male C57bl/6j Mice, supplied by clea japan inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wild+type+c57bl+6j+strain/pm40598091-38-0-6?v=clea+japan+inc
Average 90 stars, based on 1 article reviews
male c57bl/6j mice - by Bioz Stars, 2026-08
90/100 stars
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surf4 flox mice in c57bl/6j background  (BIOCYTOGEN ltd)
90
BIOCYTOGEN ltd surf4 flox mice in c57bl/6j background
( A ) Schematic of Pde6b gene correction by Cas9/RecA in <t>rd1</t> (top) and wild-type (bottom) mice. The Pde6b point mutation (C to A) is marked in red, and the premature stop codon of the Pde6b rd1 mutation is labeled by a red arrowhead. The truncated rd1 protein is in red, and the wild-type sequence loss in the rd1 mutant is in blue. ( B ) Experimental design for Cas9/RecA-mediated HDR gene correction of Pde6b rd1 mutant mice. Plasmids required for Cas9/RecA were introduced into mouse retinae by retinal electroporation. ( C ) Schematic of genomic DNA and cDNA sequencing of Cas9/RecA-treated cells. EGFP + cells from treated rd1 mice were collected using glass capillaries, with DNA extracted and enriched by Dde I restricted enzyme digestion for genome sequencing or cDNA collected through Smart-seq2 methods. ( D ) Representative direct genomic sequencing results from Cas9/RecA-mediated HDR gene correction. The Cas9-treated group (top) did not detect any gene correction. The Cas9/RecA-treated group (bottom) showed A to C conversion (red dashed frame). ( E ) Representative sequencing result of Pde6b cDNA from the Cas9/RecA-treated group. A was converted to C by Cas9/RecA-mediated gene correction. ( F ) Representative Western blot results from Cas9/RecA-mediated gene correction, and quantification of levels of PDE6B protein. Western blot for PDE6B shows the recovery of PDE6B expression. Actin was used as a loading control. Two retinae were mixed together for each Western blot sample, and three independent experiments were done. 1/10 wild type, loading 1 / 10 volume wild type and 9 / 10 sample buffer to make up the volume; Cas9, rd1 mice treated with Cas9, sgRNA, and donor; Cas9/RecA, rd1 mice treated with Cas9, sgRNA, donor, and RecA.
Surf4 Flox Mice In C57bl/6j Background, supplied by BIOCYTOGEN ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wild+type+c57bl+6j+strain/pmc10026970-16-4-9?v=BIOCYTOGEN+ltd
Average 90 stars, based on 1 article reviews
surf4 flox mice in c57bl/6j background - by Bioz Stars, 2026-08
90/100 stars
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c57bl/6j mice  (biolasco taiwan)
90
biolasco taiwan c57bl/6j mice
( A ) Schematic of Pde6b gene correction by Cas9/RecA in <t>rd1</t> (top) and wild-type (bottom) mice. The Pde6b point mutation (C to A) is marked in red, and the premature stop codon of the Pde6b rd1 mutation is labeled by a red arrowhead. The truncated rd1 protein is in red, and the wild-type sequence loss in the rd1 mutant is in blue. ( B ) Experimental design for Cas9/RecA-mediated HDR gene correction of Pde6b rd1 mutant mice. Plasmids required for Cas9/RecA were introduced into mouse retinae by retinal electroporation. ( C ) Schematic of genomic DNA and cDNA sequencing of Cas9/RecA-treated cells. EGFP + cells from treated rd1 mice were collected using glass capillaries, with DNA extracted and enriched by Dde I restricted enzyme digestion for genome sequencing or cDNA collected through Smart-seq2 methods. ( D ) Representative direct genomic sequencing results from Cas9/RecA-mediated HDR gene correction. The Cas9-treated group (top) did not detect any gene correction. The Cas9/RecA-treated group (bottom) showed A to C conversion (red dashed frame). ( E ) Representative sequencing result of Pde6b cDNA from the Cas9/RecA-treated group. A was converted to C by Cas9/RecA-mediated gene correction. ( F ) Representative Western blot results from Cas9/RecA-mediated gene correction, and quantification of levels of PDE6B protein. Western blot for PDE6B shows the recovery of PDE6B expression. Actin was used as a loading control. Two retinae were mixed together for each Western blot sample, and three independent experiments were done. 1/10 wild type, loading 1 / 10 volume wild type and 9 / 10 sample buffer to make up the volume; Cas9, rd1 mice treated with Cas9, sgRNA, and donor; Cas9/RecA, rd1 mice treated with Cas9, sgRNA, donor, and RecA.
C57bl/6j Mice, supplied by biolasco taiwan, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wild+type+c57bl+6j+strain/pm32244889-187-1-6?v=biolasco+taiwan
Average 90 stars, based on 1 article reviews
c57bl/6j mice - by Bioz Stars, 2026-08
90/100 stars
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c57bl/6j mice  (GemPharmatech Co Ltd)
90
GemPharmatech Co Ltd c57bl/6j mice
( A ) Schematic of Pde6b gene correction by Cas9/RecA in <t>rd1</t> (top) and wild-type (bottom) mice. The Pde6b point mutation (C to A) is marked in red, and the premature stop codon of the Pde6b rd1 mutation is labeled by a red arrowhead. The truncated rd1 protein is in red, and the wild-type sequence loss in the rd1 mutant is in blue. ( B ) Experimental design for Cas9/RecA-mediated HDR gene correction of Pde6b rd1 mutant mice. Plasmids required for Cas9/RecA were introduced into mouse retinae by retinal electroporation. ( C ) Schematic of genomic DNA and cDNA sequencing of Cas9/RecA-treated cells. EGFP + cells from treated rd1 mice were collected using glass capillaries, with DNA extracted and enriched by Dde I restricted enzyme digestion for genome sequencing or cDNA collected through Smart-seq2 methods. ( D ) Representative direct genomic sequencing results from Cas9/RecA-mediated HDR gene correction. The Cas9-treated group (top) did not detect any gene correction. The Cas9/RecA-treated group (bottom) showed A to C conversion (red dashed frame). ( E ) Representative sequencing result of Pde6b cDNA from the Cas9/RecA-treated group. A was converted to C by Cas9/RecA-mediated gene correction. ( F ) Representative Western blot results from Cas9/RecA-mediated gene correction, and quantification of levels of PDE6B protein. Western blot for PDE6B shows the recovery of PDE6B expression. Actin was used as a loading control. Two retinae were mixed together for each Western blot sample, and three independent experiments were done. 1/10 wild type, loading 1 / 10 volume wild type and 9 / 10 sample buffer to make up the volume; Cas9, rd1 mice treated with Cas9, sgRNA, and donor; Cas9/RecA, rd1 mice treated with Cas9, sgRNA, donor, and RecA.
C57bl/6j Mice, supplied by GemPharmatech Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wild+type+c57bl+6j+strain/10__1002_slash_adfm__202404658-312-1-6?v=GemPharmatech+Co+Ltd
Average 90 stars, based on 1 article reviews
c57bl/6j mice - by Bioz Stars, 2026-08
90/100 stars
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b6.129s1-irf4tm1rdf/j mice crossed with c57bl/6j-tg(itgaxcre,-egfp)4097ach/j mice  (Genentech inc)
90
Genentech inc b6.129s1-irf4tm1rdf/j mice crossed with c57bl/6j-tg(itgaxcre,-egfp)4097ach/j mice
( A ) Schematic of Pde6b gene correction by Cas9/RecA in <t>rd1</t> (top) and wild-type (bottom) mice. The Pde6b point mutation (C to A) is marked in red, and the premature stop codon of the Pde6b rd1 mutation is labeled by a red arrowhead. The truncated rd1 protein is in red, and the wild-type sequence loss in the rd1 mutant is in blue. ( B ) Experimental design for Cas9/RecA-mediated HDR gene correction of Pde6b rd1 mutant mice. Plasmids required for Cas9/RecA were introduced into mouse retinae by retinal electroporation. ( C ) Schematic of genomic DNA and cDNA sequencing of Cas9/RecA-treated cells. EGFP + cells from treated rd1 mice were collected using glass capillaries, with DNA extracted and enriched by Dde I restricted enzyme digestion for genome sequencing or cDNA collected through Smart-seq2 methods. ( D ) Representative direct genomic sequencing results from Cas9/RecA-mediated HDR gene correction. The Cas9-treated group (top) did not detect any gene correction. The Cas9/RecA-treated group (bottom) showed A to C conversion (red dashed frame). ( E ) Representative sequencing result of Pde6b cDNA from the Cas9/RecA-treated group. A was converted to C by Cas9/RecA-mediated gene correction. ( F ) Representative Western blot results from Cas9/RecA-mediated gene correction, and quantification of levels of PDE6B protein. Western blot for PDE6B shows the recovery of PDE6B expression. Actin was used as a loading control. Two retinae were mixed together for each Western blot sample, and three independent experiments were done. 1/10 wild type, loading 1 / 10 volume wild type and 9 / 10 sample buffer to make up the volume; Cas9, rd1 mice treated with Cas9, sgRNA, and donor; Cas9/RecA, rd1 mice treated with Cas9, sgRNA, donor, and RecA.
B6.129s1 Irf4tm1rdf/J Mice Crossed With C57bl/6j Tg(itgaxcre, Egfp)4097ach/J Mice, supplied by Genentech inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wild+type+c57bl+6j+strain/pm25907770-37-13-27?v=Genentech+inc
Average 90 stars, based on 1 article reviews
b6.129s1-irf4tm1rdf/j mice crossed with c57bl/6j-tg(itgaxcre,-egfp)4097ach/j mice - by Bioz Stars, 2026-08
90/100 stars
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Image Search Results


( A ) Schematic of Pde6b gene correction by Cas9/RecA in rd1 (top) and wild-type (bottom) mice. The Pde6b point mutation (C to A) is marked in red, and the premature stop codon of the Pde6b rd1 mutation is labeled by a red arrowhead. The truncated rd1 protein is in red, and the wild-type sequence loss in the rd1 mutant is in blue. ( B ) Experimental design for Cas9/RecA-mediated HDR gene correction of Pde6b rd1 mutant mice. Plasmids required for Cas9/RecA were introduced into mouse retinae by retinal electroporation. ( C ) Schematic of genomic DNA and cDNA sequencing of Cas9/RecA-treated cells. EGFP + cells from treated rd1 mice were collected using glass capillaries, with DNA extracted and enriched by Dde I restricted enzyme digestion for genome sequencing or cDNA collected through Smart-seq2 methods. ( D ) Representative direct genomic sequencing results from Cas9/RecA-mediated HDR gene correction. The Cas9-treated group (top) did not detect any gene correction. The Cas9/RecA-treated group (bottom) showed A to C conversion (red dashed frame). ( E ) Representative sequencing result of Pde6b cDNA from the Cas9/RecA-treated group. A was converted to C by Cas9/RecA-mediated gene correction. ( F ) Representative Western blot results from Cas9/RecA-mediated gene correction, and quantification of levels of PDE6B protein. Western blot for PDE6B shows the recovery of PDE6B expression. Actin was used as a loading control. Two retinae were mixed together for each Western blot sample, and three independent experiments were done. 1/10 wild type, loading 1 / 10 volume wild type and 9 / 10 sample buffer to make up the volume; Cas9, rd1 mice treated with Cas9, sgRNA, and donor; Cas9/RecA, rd1 mice treated with Cas9, sgRNA, donor, and RecA.

Journal: Science Advances

Article Title: In vivo genome editing rescues photoreceptor degeneration via a Cas9/RecA-mediated homology-directed repair pathway

doi: 10.1126/sciadv.aav3335

Figure Lengend Snippet: ( A ) Schematic of Pde6b gene correction by Cas9/RecA in rd1 (top) and wild-type (bottom) mice. The Pde6b point mutation (C to A) is marked in red, and the premature stop codon of the Pde6b rd1 mutation is labeled by a red arrowhead. The truncated rd1 protein is in red, and the wild-type sequence loss in the rd1 mutant is in blue. ( B ) Experimental design for Cas9/RecA-mediated HDR gene correction of Pde6b rd1 mutant mice. Plasmids required for Cas9/RecA were introduced into mouse retinae by retinal electroporation. ( C ) Schematic of genomic DNA and cDNA sequencing of Cas9/RecA-treated cells. EGFP + cells from treated rd1 mice were collected using glass capillaries, with DNA extracted and enriched by Dde I restricted enzyme digestion for genome sequencing or cDNA collected through Smart-seq2 methods. ( D ) Representative direct genomic sequencing results from Cas9/RecA-mediated HDR gene correction. The Cas9-treated group (top) did not detect any gene correction. The Cas9/RecA-treated group (bottom) showed A to C conversion (red dashed frame). ( E ) Representative sequencing result of Pde6b cDNA from the Cas9/RecA-treated group. A was converted to C by Cas9/RecA-mediated gene correction. ( F ) Representative Western blot results from Cas9/RecA-mediated gene correction, and quantification of levels of PDE6B protein. Western blot for PDE6B shows the recovery of PDE6B expression. Actin was used as a loading control. Two retinae were mixed together for each Western blot sample, and three independent experiments were done. 1/10 wild type, loading 1 / 10 volume wild type and 9 / 10 sample buffer to make up the volume; Cas9, rd1 mice treated with Cas9, sgRNA, and donor; Cas9/RecA, rd1 mice treated with Cas9, sgRNA, donor, and RecA.

Article Snippet: The rd1 (C57BL/6J), Nrl-EGFP (C57BL/6J), and cDTA (C57BL/6J) mice were gifts from K.-W. Yau (Johns Hopkins University).

Techniques: Mutagenesis, Labeling, Sequencing, Electroporation, Genomic Sequencing, Western Blot, Expressing, Control

( A ) Representative immunofluorescence images of photoreceptor markers stained in mouse retinae from wild-type, Cas9, and Cas9/RecA HDR-treated rd1 mice electroporated on P0. Green, EGFP cells from Nrl-EGFP –labeled cells; red, cone markers of swOPN and mwOPN; blue, cell nucleus shown by 4′,6-diamidino-2-phenylindole (DAPI). OS, outer segment; ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer. Arrowheads point to positive cells. Scale bars, 10 μm. ( B ) Quantification of rod photoreceptors from wild-type ( n = 3), Cas9/RecA ( n = 14), and Cas9 ( n = 7) mice electroporated on P0. ( C ) Quantification of cone photoreceptors from wild-type ( n = 3), Cas9/RecA ( n = 10), and Cas9 ( n = 5) mice electroporated on P0. *** P < 0.0001, unpaired Student’s t -test.

Journal: Science Advances

Article Title: In vivo genome editing rescues photoreceptor degeneration via a Cas9/RecA-mediated homology-directed repair pathway

doi: 10.1126/sciadv.aav3335

Figure Lengend Snippet: ( A ) Representative immunofluorescence images of photoreceptor markers stained in mouse retinae from wild-type, Cas9, and Cas9/RecA HDR-treated rd1 mice electroporated on P0. Green, EGFP cells from Nrl-EGFP –labeled cells; red, cone markers of swOPN and mwOPN; blue, cell nucleus shown by 4′,6-diamidino-2-phenylindole (DAPI). OS, outer segment; ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer. Arrowheads point to positive cells. Scale bars, 10 μm. ( B ) Quantification of rod photoreceptors from wild-type ( n = 3), Cas9/RecA ( n = 14), and Cas9 ( n = 7) mice electroporated on P0. ( C ) Quantification of cone photoreceptors from wild-type ( n = 3), Cas9/RecA ( n = 10), and Cas9 ( n = 5) mice electroporated on P0. *** P < 0.0001, unpaired Student’s t -test.

Article Snippet: The rd1 (C57BL/6J), Nrl-EGFP (C57BL/6J), and cDTA (C57BL/6J) mice were gifts from K.-W. Yau (Johns Hopkins University).

Techniques: Immunofluorescence, Staining, Labeling